Nasopharyngeal Gram-negative bacilli colonization in Brazilian children attending day-care centers

This study aimed at determining prevalence and resistance profile of Gram-negative bacilli isolated from nasopharynx of children attending day-care centers in Goiânia (Brazil). P. aeruginosa (100.0%), E. coli (50.0%), K. pneumoniae (35.3%), and E. aerogenes (16.7%) were the most frequent multi-drug resistant microorganisms isolated. No production of ESBL was detected.

Extended-spectrum -lactamases producing Klebsiella pneumoniae isolated in two hospitals in Goiânia/Brazil: detection, prevalence, antimicrobial susceptibility and molecular typing

This study was developed to evaluate the prevalence of extended-spectrum -lactamases (ESBL) producing Klebsiella pneumoniae in two hospitals (A and B) in Goiânia, GO, Brazil. The antimicrobial susceptibility of the isolates was determined using the MicroScan WalkAway (Dade Behring, USA). Tests to evaluate the genetic correlation between the isolates were also performed. For the ESBL phenotypic test, the Double-disk diffusion (DD) method was used. The strainsisolated in Hospital B were submitted to DNA analysis by pulsed-field gel electrophoresis (PFGE). The study showed high prevalence of ESBL-producing K. pneumoniae (25% in hospital A and 66.7% in hospital B), with high rates of antimicrobial resistance. The most active compound was imipenem (100% susceptibility in vitro). The PFGE test showed similiarity in five strains and variability in six strains.The high prevalence of ESBL-producing Klebsiella may be due to individual selection and to dissemination of a common strain.

Este estudo foi desenvolvido para avaliar a prevalência de Klebsiella pneumoniae produtoras de ESBLem dois hospitais (A e B) de Goiânia, GO, Brasil. Os isolados foram analisados quanto à resistência a antibióticos através do MicroScan WalkAway (Dade Behring, USA). A correlação genética entre isolados produtores de ESBL também foi avaliada. O teste fenotípico para ESBL foi realizado através de teste de Disco de Difusão Dupla (DD) e a análise do DNA de K. pneumoniae produtoras de ESBL, isoladas no Hospital B, realizada por Pulsed Field Gel Electrophoresis (PFGE). O estudo demonstrou alta prevalência de K. pneumoniae produtora de ESBL (25,0% no hospital A e 66,7% no hospital B) com altas taxas de resistência aos antimicrobianos. O composto mais ativo foi o imipenem (100% de sensibilidade in vitro). O PFGE mostrou similaridade em cinco isolados e variabilidade em seis isolados. A alta prevalência Klebsiella produtora de ESBL pode ser devida à seleção individual e à disseminação de uma cepa comum.